Everton midfielder Fabian Delph has withdrawn from the England squad and will miss the Euro 2020 qualifiers in the Czech Republic and Bulgaria.
Delph, 29, picked up a hamstring injury during Everton’s 1-0 Premier League loss at Burnley on Saturday.
A statement from the Football Association said “no further replacements are planned at this moment”.
Boss Gareth Southgate will continue his preparations with a 24-man squad.
Midfielders Dele Alli of Tottenham and Manchester United’s Jesse Lingard were among the players not selected by Southgate when the squad was announced on Thursday.
England are top of qualifying group A after four wins from four matches and face the Czech Republic away on Friday, 11 October, before playing in Bulgaria three days later.
Manchester United forward Mason Greenwood, Arsenal midfielder Reiss Nelson and Wolves midfielder Morgan Gibbs-White have all withdrawn from the England Under-21 squad to face Slovenia and Austria.
Greenwood and Gibbs-White both have back injuries and Nelson has a knee injury.
Head coach Aidy Boothroyd has called up Sam Surridge and Ben Wilmot, both on loan at Swansea, to the squad to replace the trio.
England U21s play Slovenia in Maribor on Friday before facing Austria in a 2021 European Under-21 Championship qualifier at Stadium MK on Tuesday, 15 October.